It is established that trans membrane potential (TMP) is one of the known cellular signaling pathways. TMP pathways also regulate synthesis of various proteins at the appropriate time in the living cells. Many illnesses like cancer, heart disease, degenerative disorders like cartilage loss and different kinds of inflammation are also linked to disturbances in the protein transcription process. It was postulated in 1993 that if an appropriate method can be developed to alter the TMP in a controlled manner, most of these disorders that are protein linked can be tackled.
Rotational Field Quantum Nuclear Magnetic Resonance is a technology that is made to deliver highly complex quantum nuclear magnetic resonance beams in the subradio and radio frequency spectrum (using CYTOTRONTM, developed by Centre for Advanced Research and Development (CARD), the research wing of Scalene Cybernetics Limited), with a near field delivery using specialised antenna (K- ? Ferrite type; Near Field; gain; 10dB), that when appropriately controlled can alter the TMP to the specific requirement like stimulation of HSP 70 group of protein in cartilage regeneration or P53 and its silent partner P73 protein in case of neoplastic disorders (malignant tumors). The delivered instantaneous mode magnetic and RF modulation is highly cell specific (based on proton density, permitivity and depth of penetration), to modulate the cell membrane potential in vivo, such that specific protein required for tissue regeneration or degeneration is initiated.
In chondrogenesis (cartilage cells) the Rotational Field Quantum Nuclear Magnetic Resonance beams are precisely controlled and focussed onto tissues therein generating streaming voltage potentials. This flow in the joint causes forced movement of hydrogen protons in the extra-cellular matrix (ECM) due to alteration in nuclear spin in the hydrogen atoms and subsequent resonance. This causes a decrease in the TMP of the chondrocytes stimulating the production of the required protein for initiation of mitosis by dedifferentiation and redifferentiation.
In a study to establish tissue regeneration, 202 patients with confirmed diagnosis of osteoarthritis of knee joint ( M : F; 65:137), mean age: 57 ? 28) who were advised total knee replacement were recruited. These patients were treated with Rotational Field Quantum Nuclear Magnetic Resonance focussed on both knees for thirty minutes every day for 21 consecutive days. They were evaluated before, and three month post-treatment with the Knee Society Scoring System, dynamometry and MRI evaluation of the knee joint using 3D FLASH sequence with water excitation. There was highly significant improvement in Pain Score, Total Knee Score (TKS), Total Functional Score (TFS), Range of Movement (ROM) and force of extension, immediately after the treatment. This improvement persisted when evaluation was repeated after three months. Three-month post-treatment showed that there was a significant increase in cartilage thickness (CT) as established by MRI. The summarised results are shown in the table.
Values are Mean ? SEM * represents p
In conclusion, use of Rotational Field Quantum Nuclear Magnetic Resonance technique operating at low end spectrum radio frequency can alter the TMP to stimulate and regenerate cartilage tissue in vivo and can be of great help in patients suffering from osteoarthritis of any joint.
(The writers can be contacted at Centre for Advanced Research and Development (CARD), Bengaluru, and CLUMAX Diagnostic and Research Centre (CDRC), Bengaluru.)